Journal: Frontiers in Synaptic Neuroscience

Article Title: BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons

doi: 10.3389/fnsyn.2014.00027

Figure Lengend Snippet: Both evoked and spontaneous uptake of anti-SYT1 antibody is enhanced by BDNF treatment in excitatory but not inhibitory terminals. (A) Schematic of the anti-SYT1 antibody uptake experiment. (B) Representative immunofluorescence images of evoked and spontaneous anti-SYT1 antibody uptake in excitatory terminals. (C) Statistical analysis of normalized fluorescence intensity of both evoked and spontaneous anti-SYT1 antibody uptake in control (white), 5 min (gray) and 48 h (black) BDNF treatments in vGluT1 positive terminals. (D) Ratio of evoked and spontaneous anti-SYT1 antibody uptake. (E) Representative immunofluorescence images of evoked and spontaneous anti-SYT1 antibody uptake in inhibitory terminals. (F) Statistical analysis of both evoked and spontaneous anti-SYT1 antibody uptake in inhibitory terminals in control (white), 5 min (gray) and 48 h (black) BDNF treatments. (G) Ratio of evoked and spontaneous anti-SYT1 antibody uptake. Scale bars = 5 µm.

Article Snippet: Neurons were incubated with a guinea pig polyclonal anti-SYT1 antibody (Synaptic Systems) at 1:800 in 47 mM KCl (evoked) or 4 mM KCl (basal) Tyrode solution for 5 min, and then in basal Tyrode solution containing the same antibody concentration for 5 additional min. After washing with basal Tyrode solution for 10 min, a mouse monoclonal anti-SYT1 antibody (Synaptic Systems) was loaded in the presence of 1 μM Tetrodotoxin (TTX) in basal Tyrode solution for 10 min for spontaneous uptake.

Techniques: Immunofluorescence, Fluorescence, Control

Journal: The Journal of comparative neurology

Article Title: Three-Dimensional Ultrastructure of Flower-Spray Nerve Endings in the Rat Carotid Sinus.

doi: 10.1002/cne.25654

Figure Lengend Snippet: FIGURE 1 Whole mount preparation of the carotid sinus with double immunofluorescence for P2 × 3 (green) and Syt1 (red). (a) Low magnification view of the carotid sinus showing P2 × 3-immunoreactive flower-spray nerve endings. Syt1 immunoreactivity is shown in flower-spray endings and in the network of varicose nerve fibers. (b and c) Three-dimensional view of the basal surface of the terminal part of the flower-spray ending indicated in rectangle in Panel a. Arrows indicate thick parent axon for the ending. Panel b shows flower-spray endings could be distinguished from network of thin varicose nerve fibers with Syn1 immunoreactivity. (d–f) Higher magnification view of the rectangle in Panel a. (d) Punctate P2 × 3 immunoreactivity is shown in the terminal part of the endings. (e) Syt1 immunoreactivity is shown as smaller dots in the terminal parts. (f) The merged figure shows that P2 × 3 and Syt1 immunoreactivities are distinct from each other.

Article Snippet: A mouse monoclonal anti-Syt1 antibody (clone ASV48, MAB4364, R&D Systems, Minneapolis, MN, USA; RRID AB_2199304) was raised against the rat brain synaptic plasmamembrane (Matthew, Tsavaler, and Reichardt 1981).

Techniques: Immunofluorescence

Journal: eLife

Article Title: Ca 2+ sensor synaptotagmin-1 mediates exocytosis in mammalian photoreceptors

doi: 10.7554/eLife.45946

Figure Lengend Snippet:

Article Snippet: Antibody , Mouse anti-Syt1*Oyster550 , Synaptic Systems , 105 011C3; RRID: AB_887827 , 1:1000.

Techniques: Plasmid Preparation